Bone tissue regeneration using 3D printed microstructure incorporated with hybrid nano hydrogel

... we have successfully designed and developed a novel functional hydrogel incorporating cRGD conjugated GNPs (RGNPs), which reinforced the mechanical strength through the combination of 3D printed microstructure and hydrogel. The stiffness of the composite microstructure was significantly increased to a similar level of native bone tissue. Before cell encapsulation, the cell viability tests suggest that all components not only had no significant cytotoxic effects but also, RGNPs showed and increased cell proliferation with dose-dependent manner. Moreover, the addition of RGD to the composite constructs enhanced the cellular behavior. The in vitro experiments showed that encapsulated ADSCs in the Gel- RGNPs promoted significantly higher cell adhesion, spreading and proliferation, which led to an increased gene expression of osteogenic specific markers. Our findings suggest that reinforced composite hydrogels can be a useful strategy for improving the bone tissue regeneration.

The poster is here: L0K28001-Bone tissue regeneration using 3D printed microstructure incorporated with hybrid nano hydrogel.pdf