Enhanced neural stem cell differentiation in 3D printed scaffolds with light stimulation

... This study shows that neural lineage of NSCs on 3D printed scaffold respond very favorably to LLLT, and can be coaxed to increase cell proliferation rate. ROS synthesis of NSCs was also promoted by laser stimulation. When NSCs were cultured on 3D printed scaffold and simulated by laser, it was demonstrated that the expression of glial cell marker was suppressed while positive neuronal population of cells was significantly enhanced. Gene expression levels of neuronal makers were also upregulated for the cells stimulated with laser, indicating the laser stimulation improved neuronal differentiation of NSCs. 3D printing technology is superior to many other conventional scaffold fabrication approaches regarding the design and controllability of architecture, and LLLT is a powerful adjunct strategy for improved cell function. Therefore, the integration of 3D printing and LLLT might provide a new methodology for neural regeneration.

The poster is here: L0K17002-Enhanced neural stem cell differentiation in 3D printed scaffolds with light stimulation.pdf